Inhibitory effect induced by docetaxel, gefitinib, and NS-398 on the invasive potential of PC3M and DU-145 cells.

<p>The cells were untreated (control) or exposed for 24 h to 0.005 μmol/L docetaxel (D), 10 μmol/L gefitinib (G) or 50 μmol/LNS-398 (N), alone or in combination during in vitro invasion assays, performed using transwell bicameral chambers as described in Materials and Methods. At the end of the 24 h assay, the invading cells were stained and counted by phase-contrast microscopy (x 200). Representative results show the mean number of cells invading through the Matrigel insert membrane in five random microscope fields. *P <0.05, ** P <0.005, ***, P <0.001 compared with control values.