Deletion of the <i>trx2</i>⁺ gene enhanced Rst2 transcriptional activity.

<p>(A) The Δ<i>trx1</i> and Δ<i>trx2</i> cells showed H₂O₂-sensitive phenotype. Wild-type, Δ<i>trx1</i> and Δ<i>trx2</i> cells were streaked onto YES plates with or without 3 mM H₂O₂, and cultured at 30°C for 3 days. (B) Deletion of the <i>trx2</i>⁺ gene enhanced Rst2 transcriptional activity. Wild-type and Δ<i>trx2</i> cells harboring the reporter plasmid were cultured and assayed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078012#pone-0078012-g001" target="_blank">Figure 1A</a>. (C and D) Deletion of the <i>trx2</i>⁺ gene specifically enhanced Rst2 transcriptional activity. Wild-type, Δ<i>trx2</i>, Δ<i>tpx1</i> and Δ<i>pap1</i> cells harboring the reporter plasmid were cultured and assayed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078012#pone-0078012-g001" target="_blank">Figure 1A</a>. Error bars, mean ± S.D. (n ≥ 3).