NO may be involved in the activation of Rst2.

<p>(A and B) NO generators activated Rst2 transcriptional activity. Wild-type cells harboring the reporter plasmid were treated with GR or GD in the presence or absence of 125 μM NO generators (SNAP, DEA NONOate or SNP). (C and D) Calboxy-PTIO inhibited Rst2 transcriptional activity upon glucose deprivation. Wild-type cells harboring the reporter plasmid were treated with GD in the presence or absence of calboxy-PTIO (0.125 mM to 1 mM). Error bars, mean ± S.D. (n ≥ 3).