DAT levels in D5R-KO mice.

<p>(a) Dialysate samples were collected at a sampling rate of 2 µl/min for 20 min during a baseline period of 60 min and then for an experimental period of 120 min following the METH challenge (2.5 mg/kg; arrow). The interaction between blocker pretreatment and challenge was F_(5,120) = 6.66 and <i>p</i><0.0001. The simple main effects of blocker pretreatment at 40 and 60 min were F_(1,144) = 13.50 and 8.08, and <i>p</i><0.001 and <i>p</i><0.001, respectively. WT, filled circles (<i>n</i> = 7); D5R-KO, open circles (<i>n</i> = 7). DA, dopamine. The arrowhead indicates the time point of saline injection. (b) Dialysate samples were collected for 20 min during a baseline period of 60 min. The animals were then injected with GBR12909 (5 mg/kg) and samples were collected for 20 min during a pretreatment period of 80 min. The animals were then challenged with METH (2.5 mg/kg) and samples were collected throughout the 120 min experimental period. The arrowhead indicates the GBR12909 pretreatment and the arrow indicates the METH challenge. (c) Representative microdialysis probe placements. Dashed lines denote the boundaries of the NA and the anterior commissure (ac). (d) Schematic representations of probe placements in the experimental groups for microdialysis of the NA at two different rostrocaudal levels (1.1 mm and 0.8 mm rostral from the bregma). The short lines indicate the probe tracks. Seven WT and seven D5R-KO probe track cases are overlaid on representative sections.