Thick section photomicrographs and ultrathin electron micrographs of OHSU_I and OHSU_II microbes.

<p><b>A and B:</b> Thick section photomicrographs of OHSU_I C6 (A) and OHSU_II C1 (B) that were concentrated from cultures using modified SP4 broth. Both sections revealed morphologically similar, slender pointed microbes using longitudinal and cross sectioning. The concentrated microbes were fixed with 2.5% glutaraldehyde, post-fixed with 1% osmium tetroxide and embedded in epoxy resin. The thick sections were stained using 1% toluene blue. 1000X. <b>C and D:</b> Electron photomicrographs of OHSU_I C6 (C) and OHSU_II C1 (D) captured in ultrathin sections. Typical Gram-negative bacteria wall structure (arrows), intracellular ribosomal structures (R), electron-dense bodies and nucleic acid (N) as well as scale bars are indicated for both sections. The ultrathin sections were stained with uranyl acetate and lead citrate. <b>E:</b> Electron micrograph of OHSU_I C6 captured at higher magnification (200,000X). External membrane (black arrow) and internal membrane structures (red arrow) of the microbes are highlighted.