The influence of COS on PQ-induced cell death, apoptosis and antioxidative enzyme in RGC-5 cells.

<p>(A) Effects of COS on RGC-5 viability determined by MTT assays. RGC-5 cell were incubated for 24 h with vehicle or COS at indicated concentrations. (B) COS decreased PQ-stimulated RGC-5 cell death. RGC-5 cell were stimulated with 0.5 mM PQ and treated with 50 or 100 μg/ml COS for indicated time. (C) Inhibition by COS of PQ-induced apoptosis. RGC-5 cell were stimulated with 0.5 mM PQ and treated with 50 or 100 μg/ml COS or PBS for 6 hours. Cells apoptosis was analyzed by TUNEL stain. Percent apoptotic RGC-5 cells were obtained by counting at least 400 cells. Data were expressed as mean ± SD. ns, Non-significant compared with vehicle-treated control. (*<i>P</i> < 0.05 compared with PQ only or PBS-treated group; # <i>P</i> < 0.05 compared with normal; & <i>P</i>< 0.05 by ANOVA with post hoc Bonferroni test; N=3 in each group) (D) COS activated catalase activity in PQ-stimulated RGC-5. RGC-5 cell were stimulated with 0.5 mM PQ and treated with 100 μg/ml COS for indicated time. (E) COS increased GSH levels in PQ-stimulated RGC-5. (†<i>P</i> < 0.05 compared with PQ only by Student’s t-test; N=3 in each group).