OTUD5 interacts with p53.

<p>(A) OTUD5 interacts with p53 in yeast two-hybrid assay. AH109 yeast cells were cotransformed with indicated plasmids, then plated on SD/–Ade/–His/–Leu/–Trp selection mediums. (B) The HEK293 cells were cotransfected with Flag- OTUD5 and Myc-p53, and cell lysates were immunoprecipitated with indicated antibodies and immunoblotted with anti-Myc or anti-Flag antibody. (C) Immunoprecipitation of p53 from U2OS cells, followed by western blotting of the precipitated proteins with anti- OTUD5, anti-p53 and anti-MDM2 antibodies. (D)Pull-down assay of His-tagged OTUD5 by GST fusion p53 protein bound to GSH-agarose beads, and subsequent detection by western with anti-His antibody. (E) Immunofluorescent detection of Flag-OTUD5 and Myc-p53. Nuclei are counterstained by DAPI.