DNA binding ability and helicase activity of drUvrD mutants.

<p>Comparison of DNA binding ability and helicase activity of wild type (WT) and <i>dr</i>UvrD mutants: β-hairpin deletion mutant (ΔHairpin), and mutants of the GIG motif from domain 2B involved in dsDNA binding (G424T, G426T and double mutant G424T/G426T). A. DNA binding affinities (K_d values) of WT and mutant <i>dr</i>UvrD for either 3'-tailed (blue) or 5'-tailed (red) dsDNA determined by fluorescence anisotropy measurements. B. Helicase activity of WT and mutant <i>dr</i>UvrD (250 nM) on 3'-tailed (blue) or 5'-tailed (red) dsDNA (20 nM). Initial reaction rates were determined from reaction time courses and were normalized with respect to the activity of WT <i>dr</i>UvrD. Standard deviations are shown as vertical bars.