MADM-ML has significantly higher recombination efficiency than MADM-SL, and maintains the productive G2-X segregation pattern.

<p>(A) Representative images of cerebella in MADM Math1-Cre mice at P5 and P14 with quantification areas outlined (EGL for P5, IGL for P14). MADM-ML has significantly more labeled cells than MADM-SL does, for both P5 and P14 data set. P5 data (n=5 for ML/ML, n=4 for SL/SL, and n=5 ML/SL) are analyzed by ANOVA and multiple comparisons with Tukey HSD. There is no significant difference between SL/SL and ML/SL. P14 data (n=5 for ML/ML and n=7 for SL/SL) are analyzed by an independent samples t-test with unequal variance. (B) There is no significant difference in G2-X segregation frequency between the two MADM systems, as measured by percent of green and red cells among total labeled cells. n=5 for ML/ML and n=7 for SL/SL. Error bars are one standard deviation.