The generation of a medulloblastoma model with the MADM system.

<p>(A) Breeding scheme for generating medulloblastoma mouse model. (B) The correlation between fluorescent protein labeling and genotype of cells generated by MADM in this model. All cells in the animal are heterozygous for <i>Ptc</i> mutation but <i>p53</i> status varies. Green cells are homozygous null for <i>p53</i>; red cells are homozygous WT for <i>p53</i>; yellow and colorless cells are heterozygous for <i>p53</i> (KO/WT). (C) Left, a normal adult brain contains a floxed-stop Rosa-tdTomato Cre reporter and <i>Math1</i>-Cre. Middle, CKO medulloblastoma model contains the same Cre line and reporter, heterozygous <i>Ptc</i> mutation, and homozygous <i>p53flox</i> alleles. Right, an early tumor formed using our MADM mudulloblastoma model. T: tumor. Scale bar: 500um.