Binding of MS17-57 to purified GI cancer markers and lysates of fresh tissues and cells.

<p>ELISA results showed that MS17-57 bound to lysates of fresh GC tissues (strong binding in lysate from one patient and moderate binding in lysate from another patient) and to lysates of GC MKN45 cells, but not to fresh lysates of adjacent noncancerous tissues from the same patients. MS17-57 bound slightly to the purified CA15-3 protein but not to proteins or lysates of PG-1, PG-2, CEA, or <i>H. pylori</i>. ELISA used two dose-dependent dilutions of antibodies. Normal mouse serum protein was used as a negative control.