Regulation of miRs in PDG-treated trophoblast cells.

<p>TLR6^- or TLR6⁺ trophoblast cells were treated with no treatment (NT) or PDG (80μg/ml) for 12h, after which RNA was collected and the expression of: (A) miR-329; (B) miR-23a; (C) miR-let-7c; (D) miR-149; and (E) miR-23b was measured by qRT-PCR. Data are presented as fold change (FC) in miR expression after normalization to the endogenous control, miR-374. PDG treatment significantly upregulated trophoblast miR-329, miR-23a, miR-let-7c, and miR-23b expression in TLR6^-, and this was significantly inhibited by the presence of TLR6 (TLR6⁺). *<i>p</i><0.05 relative to the NT control unless otherwise indicated. Data are from 5-7 independent experiments.