Expression of miR-9 and its precursors is reduced in patient neurons under stress.

<p>(<b>A</b>) Relative expression levels of mature miR-9 in neurons derived from two control and three patient iPSC lines containing the A90V mutation with or without STS treatment (left panel). The averages in control and patient neurons are shown on the right. (<b>B</b>) Expression of pre-miR-9. (<b>C</b>) Expression of pri-miR-9-2. The average of different lines from the same individual is set as 1. The results in A–C were confirmed from additional two independent neuronal differentiation experiments. (<b>D</b>) The relative expression levels of pri-miR-9-2 and pre-miR-9-2 in neurons derived from two patient iPSC lines containing the <i>TARDBP</i> M337V mutation was reduced. This result is based on two independent differentiation experiments. (<b>E</b>) TDP-43 levels were greatly decreased in primary mouse neurons transfected with two <i>TARDBP</i> shRNAs. (<b>F</b>) Expression of pri-miR-9-2 is decreased in primary mouse neurons transfected with <i>TARDBP</i> shRNA. Values are mean ± SEM. **: <i>p</i><0.01 (Student's t-test).