<div><p>Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted substrates through the highly conserved catalytic domain. Our previous computational studies proposed a model stating that a properly assembled nonlinear motif termed the Regulatory (R) spine is essential for catalytic activity of EPKs. Here we define the required intramolecular interactions and biochemical properties of the R-spine and the newly identified “Shell” that surrounds the R-spine using site-directed mutagenesis and various <i>in vitro</i> phosphoryl transfer assays using cyclic AMP-dependent protein kinase as a representative of the entire kinome. Analysis of the 172 available Apo EPK structures in the protein data bank (P...
Thesis (Ph.D.)--University of Washington, 2019The majority of the human proteome is phosphorylated b...
In recognition of the first protein kinase structure that was solved 25 years ago, we review the his...
1. The Src-family protein tyrosine kinases (SFKs) are multidomain oncogenic protein tyrosine kinases...
Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted ...
Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted ...
Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted ...
Eukaryotic protein kinases (EPKs) regulate almost every biological process and have evolved to be dy...
The eukaryotic protein kinases (ePKs) constitute one of the largest families of enzymes encoded by e...
Eukaryotic protein kinases (EPKs) constitute a class of allosteric switches that mediate a myriad of...
Protein kinases have evolved in eukaryotes to be highly dynamic molecular switches that regulate a p...
Eukaryotic protein kinases regulate most cellular functions by phosphorylating targeted protein subs...
Statistical analysis of the functional constraints acting on eukaryotic protein kinases (EPKs) and o...
Protein kinases are key components in cellular signalling pathways as they carry out the phosphoryla...
<p>(A) The conserved EPK structural core is shown mapped on the catalytic subunit of PKA (PDB ID: 1A...
Eukaryotic protein kinases are fundamental factors for cellular regulation and therefore subject of ...
Thesis (Ph.D.)--University of Washington, 2019The majority of the human proteome is phosphorylated b...
In recognition of the first protein kinase structure that was solved 25 years ago, we review the his...
1. The Src-family protein tyrosine kinases (SFKs) are multidomain oncogenic protein tyrosine kinases...
Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted ...
Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted ...
Eukaryotic protein kinases (EPKs) regulate numerous signaling processes by phosphorylating targeted ...
Eukaryotic protein kinases (EPKs) regulate almost every biological process and have evolved to be dy...
The eukaryotic protein kinases (ePKs) constitute one of the largest families of enzymes encoded by e...
Eukaryotic protein kinases (EPKs) constitute a class of allosteric switches that mediate a myriad of...
Protein kinases have evolved in eukaryotes to be highly dynamic molecular switches that regulate a p...
Eukaryotic protein kinases regulate most cellular functions by phosphorylating targeted protein subs...
Statistical analysis of the functional constraints acting on eukaryotic protein kinases (EPKs) and o...
Protein kinases are key components in cellular signalling pathways as they carry out the phosphoryla...
<p>(A) The conserved EPK structural core is shown mapped on the catalytic subunit of PKA (PDB ID: 1A...
Eukaryotic protein kinases are fundamental factors for cellular regulation and therefore subject of ...
Thesis (Ph.D.)--University of Washington, 2019The majority of the human proteome is phosphorylated b...
In recognition of the first protein kinase structure that was solved 25 years ago, we review the his...
1. The Src-family protein tyrosine kinases (SFKs) are multidomain oncogenic protein tyrosine kinases...