Temporal expression of Pelp1 and bone markers in rBMSCs during osteogenic differentiation.

<p>rBMSCs were cultured in osteogenic differentiation culture for 1, 3, 5, 7. 9. 11. 14, and 21 days. mRNA expressions of ALP (A), OCN (B) and Pelp1 (C) were examined by real-time RT-PCR. β-actin was used as an internal control. (D) Protein expression of Pelp1 during osteogenic differentiation was examined by western blotting. β-actin was used as an internal control. The mRNA and protein expressions at 1 day of culture were normalized to “1”. Data represent the mean ± SD of three independent experiments. *<i>P</i><0.05 <i>vs</i>. baseline value (day 1).