Knockdown of PODXL expression correlates with decreased cell proliferation.

<p>(A): Lentiviral knockdown of PODXL expression, as assayed by flow cytometry, using the two shRNA sequences utilized for functional studies. (B): Representative growth curve of PODXL knockdown with TRCN0000117018 and control knockdown in JHU-0879 cells over 7 days and (C) the linear regression fit of the growth curves (p&lt;0.0001). Quantitatively similar results were obtained with each replicate. (D): Analytical flow cytometry histogram of JHU-0879 cells transduced with either control virus (red) or TRCN0000117018 virus (blue), demonstrating the knockdown of PODXL expression. (E): Representative growth curve of PODXL knockdown with V2THS_36258 and control knockdown in JHU-0879 cells over 7 days and (F) the linear regression fit of the growth curves (p = 0.0092). Quantitatively similar results were obtained with each replicate.