Expression of miR-4772-5p-iso in CD14+ monocytes between sepsis and SIRS compared with CD14 depleted PBMCs, and expression levels in healthy monocytes stimulated by TLR ligands.

<p>(<b>A</b>) Small RNA sequencing of CD14+ monocytes vs CD14 depleted PBMCs for miR-4772-5p-iso expression in Healthy, Sepsis, and SIRS samples (experiments were performed using RNA from a pool of samples in each group, see Table S1.). (<b>B</b>) Fold changes of sequencing results based on the number of reads/million in Sepsis vs SIRS group; (<b>C</b>) <b>qRT-PCR results of</b> microRNA miR-4772-5p-iso expression in CD14+ monocytes from healthy donors stimulated with different TLR ligands for 24 hours; does and source of TLR ligands listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075918#s2" target="_blank">methods</a> section. qRT-PCR was done using triplicates. Fold changes were calculated based on RPMI medium alone (see method) then normalised to percentage of maximum effect in each experiment, error bars represents Standard Error of the Mean (SEM); n = 2.