Modulatory effects of Ashwagandha and β- Amyloid_1-42 on human neuronal cells.

<p>A. Dose-response curve of Ashwagandha showing optimal concentrations and B. Dose-response cytotoxic effects of β-amyloid_1-42 on SK-N-MC cells. Cells were treated with different concentrations of Ashwagandha / β-amyloid_1-42 and mitochondrial function was determined by the MTT reduction assay as described in the Materials and Methods. C. MTT assay showing the inhibition of cell viability by β- Amyloid_1-42 (βA) and its reversal by Ashwagandha (ASH) at different concentrations on SK-N-MC cell line D. Effect of β-amyloid_1-42 on LDH leakage in SK-N-MC cells and its reversal by Ashwagandha. The data are expressed as mean ± SD of four independent experiments. (*) indicates a statistically significant difference compared to controls (p<0.05).