CST regulates the nuclear accumulation of DAF-16.

<p>(A) <i>cst-1</i> RNAi diminished DAF-16 nuclear translocation by <i>D. coniospora</i> infection (DC) and treatment with spiny balls (SB). n = 100–110 nematodes per condition. These results are means±SD of four experiments. *<i>P<</i>0.05 <i>versus</i> control (DC and SB). (B) Knock-down of <i>itr-1</i> inhibited the expression of <i>Psod-3::GFP</i> induced by <i>D. coniospora</i> infection and spiny balls for 12 h. *<i>P<</i>0.05 <i>versus</i> control (N2). (C and D) <i>cst-1</i> RNAi reduced the survival rate of nematodes exposed to <i>D. coniospora</i> (C) and spiny balls (D). <i>P</i><0.001 relative to control with empty vector. However, <i>cst-1</i> RNAi did not enhanced susceptibility of <i>daf-16(mu86)</i> mutants to killing by <i>D. coniospora</i> (C) and spiny balls (D). (E and F) Epidermal-specific RNAi of <i>cst-1</i> significantly reduced the survival rate of worms exposed to <i>D. coniospora</i> (E) and spiny balls (F). <i>P</i><0.001 relative to control with empty vector.