Involvement of cannabinoid receptors in gp120-induced suppression of DA uptake and apoptosis.

<p>Human mesencephalic neuronal/glial cultures were untreated (C) or pretreated with SR141716A (CB₁ antagonist) or SR144528 (CB₂ antagonist) (1 μM) for 30 min prior to WIN55,212-2 (0.3 μM) or JWH015 (CB₂ agonist) (1 μM) addition for 3h followed by gp120 (10 nM) treatment for 5 days to measure <b>A</b>) ³H-DA uptake and <b>B</b>, <b>C</b>) apoptosis by cell death ELISA. Data are mean ± SEM of triplicates of 2-4 separate experiments using different brain tissue specimens. **p<0.01 vs. control; ††p<0.01 vs. gp120; §§p<0.01 vs. WIN55,212-2+gp120 (in A, B) or JWH015+gp120 (in C); p<0.05 between (SR144528+JWH015+gp120) vs. (SR141716A+JWH015+gp120) (in C).