Diminished pulmonary leukocyte influx and IL1β production in glyburide-treated mice.

<p>Note. BALF = bronchoalveolar lavage fluid; IL1β = interleukin-1beta. Mice were treated with glyburide or vehicle for seven days prior to inoculation with ∼6×10² cfu <i>B. pseudomallei</i>. All mice were treated with ceftazidime starting 24 h after inoculation until sacrifice (eight animals per group per time point). Error bars indicate standard deviations. A single <i>p</i>-value is reported unless there is evidence from a test of interaction that effects at each time point are different. <b>A.</b> No differences were found in wet weight of lung or in protein infiltration. <b>B.</b> Cellular infiltrate was reduced in glyburide-treated mice compared to untreated controls at the 48-hour time point and this difference was seen in both neutrophil and monocyte fractions. <b>C.</b> IL1β concentrations were lower in glyburide-treated mice compared to untreated controls at the 48-hour time point. This difference was greater in BALF than in lung tissue.