Positional cloning of the <i>HOS5</i> gene and complementation of the <i>hos5-1</i> mutant.

<p>(A) <i>HOS5</i> is localized on Chromosome V. Schematic structure of the <i>HOS5</i> gene. Black boxes, exons; white boxes, UTRs; lines, introns; star, the <i>hos5</i>-<i>1</i> mutation site. (B) Structure of HOS5 protein and position of <i>hos5-1</i> mutation. (C–F) Complementation of the <i>hos5-1</i> phenotype by the <i>HOS5</i> gene (C), allelism testing (D–E), and segregation of a F₂ population (F). Ten-day-old seedlings grown in a ½ MS (Murashige and Skoog) agar medium were treated with 300 mM NaCl for 3 hr before imaging with a CCD camera. Shown are morphology of the seedlings and luminescence images. C24, the wild type (with <i>RD29A-LUC</i> reporter); <i>gHOS5:hos5-1, hos5-1</i> plants expressing the wild-type <i>HOS5</i> genomic DNA; <i>hos5</i>-<i>1</i>×<i>hos5</i>-<i>2</i>, F₁ progeny of a cross between the two mutants; <i>hos5</i>-<i>1</i>×<i>hos5</i>-<i>3</i>, F₁ progeny of a cross between the two mutants; <i>hos5-2</i>×C24 F₂, segregated population of the F₂ generation of a cross between <i>hos5-2</i> and wild type C24.