Mycobacteria specific intracellular IFN-γ production in CD4⁺ and CD8⁺ T spleen cells of mice vaccinated with MGM1991, HMA or BCG vaccine at 4 weeks after immunization.

<div><p><u>Upper </u><u>panel </u>(A): ICS staining of CD4⁺ spleen cells from C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice. Mean % of positive cells in each quadrant (of 3 mice tested individually in each group).</p> <p><u>Lower 2 </u><u>panels </u>(B): ICS staining of CD8⁺ spleen cells from C57BL/6 mice vaccinated 4 weeks before with 5x10⁴ CFU of MGM1991, HMA or <i>M. bovis</i> BCG by the subcutaneous route or from unvaccinated (naïve) mice. Results show results obtained in 2 individual mice.</p> <p>^*<i>p</i><0,05 MGM1991 or HMA vs BCG; ^Ŧ<i>P</i><0,05 MGM1991 vs HMA; (Mann-Whitney test).</p>