Cells with impaired function of the Ssa2p•Mas5 chaperone complex exhibit elevated levels of heat-stress associated transcription.

<p>(<b>A</b>) Top panel represents correlation analysis between gene expression profiles of <i>mas5Δ</i> (in blue) or <i>ssa2Δ</i> (in magenta) cells and wild type cells exposed to heat stress. Individual dots represent individual genes. Pearson's correlation coefficients and statistical significance are indicated. Venn diagrams (bottom panel) analyze the overlap in >2-fold differentially regulated genes between <i>mas5Δ</i> (in blue), <i>ssa2Δ</i> (in magenta) cells and heat-stressed wild type cells (in gray). (<b>B</b>) Comparison between gene expression profiles of <i>mas5Δ</i>, <i>ssa2Δ</i> and wild type cells under indicated environmental stresses; gene clustering presented on the left. (<b>C</b>) Expression profiles of genes specifically upregulated by distinct environmental stresses in <i>mas5Δ</i> and <i>ssa2Δ</i> cells and wild type cells under indicated environmental stresses. (<b>D</b>) Analysis of expression of common environmental stress response genes in exponentially growing <i>mas5Δ</i> and <i>ssa2Δ</i> cells, and <i>sty1Δ</i>, <i>atf1Δ</i> and wild type cells under indicated conditions. (<b>E</b>) Left panel shows the comparison between gene expression profiles of wild type heat-stressed cells, <i>mas5Δ</i>, <i>ssa2Δ</i> and cells overexpressing Hsf1, as detected by RNAseq analysis. Venn diagrams (right panel) analyze the overlap in >2-fold differentially regulated genes between <i>mas5Δ</i> (in blue), <i>ssa2Δ</i> (in magenta) cells, heat-stressed wild type cells (in gray) and cells overexpressing Hsf1 (in green).