HIV-1<sub>BaL</sub>(CD45<sup>−</sup>) is more infectious in MDDCs than HIV-1<sub>BaL</sub>(pellet).
- Sarah K. Mercier (473698)
- Heather Donaghy (473699)
- Rachel A. Botting (473700)
- Stuart G. Turville (51205)
- Andrew N. Harman (473701)
- Najla Nasr (473702)
- Hong Ji (293468)
- Ulrike Kusebauch (473703)
- Luis Mendoza (2090)
- David Shteynberg (473704)
- Kerrie Sandgren (473705)
- Richard J. Simpson (268613)
- Robert L. Moritz (473706)
- Anthony L. Cunningham (161201)
DOIAbstract
<p>MDDCs were infected for 48-1<sub>BaL</sub>(pellet) or HIV-1<sub>BaL</sub>(CD45<sup>−</sup>) at an MOI of 3 determined by TCID<sub>50</sub>, and assessed for intracellular HIV-1 p24 antibody by flow cytometry and HIV-1 proviral DNA by Q-PCR. A) Representative dot plots of untreated and HIV-1<sub>BaL</sub>(CD45<sup>−</sup>) exposed DCs identifying infected (p24<sup>+</sup> cells in box on the right) and exposed but non-infected (p24<sup>−</sup>) DCs at 120 hours. B) The level of infection at 48 and 120 hours for both HIV-1<sub>BaL</sub>(pellet) and HIV-1<sub>BaL</sub>(CD45<sup>−</sup>) viruses by flow cytometry (mean +/− SEM, n = 12, *p<0.05 **p<0.01) and C) by Q-PCR (HIV-1 proviral DNA normalised by albumin values) (mean +/− SEM, n = 4, *...
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