The effect of Pam3CSK4 and LPS on PLK activation and expression .

<p>(A, B) Pam3CSK4 and LPS induce dose-dependent activation of PLK1. THP-1 cells were treated with the indicated concentrations of Pam3CSK4 (A) or LPS (B) for 30 min. The phosphorylation of PLK1 was detected by western blot. β-actin protein was detected as loading control. ‘+’ indicated the non-specific bands. (C, D) Pam3CSK4 and LPS induced time-dependent activation of PLK1. The THP-1 cells were treated with 1 µg/ml Pam3CSK4 (C) or LPS (D) for the indicated time periods. The phosphorylation of PLK1 was detected by western blot. β-actin protein was detected as loading control. ‘+’ indicated the non-specific bands. (E) The effect of Pam3CSK4 and LPS on PLK gene expression. THP-1 cells were treated with 1 µg/ml Pam3CSK4 or LPS for 24 h. The gene expression of <i>PLKs</i> was detected by qRT-PCR. The <i>PLK1</i> gene expression in non-treated group was designated as 1-fold. The <i>PLK1</i> gene expression in Pam3CSK4- or LPS-treated groups, and the <i>PLK2-4</i> gene expression in all groups was shown relative to the <i>PLK1</i> gene expression in the non-treated group. * <i>P</i> < 0.05 compared with the control groups respectively.