Quantitation of HERV-K expression by qRT-PCR in 12 cancer cell lines after irradiation.

<p>Genomic positions of the primers used are shown in Fig. 1. Five different ionizing radiation doses were used (0, 2.5, 5, 10, and 20 Gy), each applied in a single dose, are shown in different colors at five different times following the γ-irradiation (1, 3, 8, 24, and 72 hours). Fold change relative to 0 Gy for each time point was obtained by normalization to 3 housekeeping genes. Bars represent the means of three independent RT-PCR replicates performed for each of three experiments. Error bars show standard deviations.