Recovery of cfDNA and miRNA from plasma: effects of increasing centrifugation speed.

<p>A. Quantitation of cfDNA using qPCR for <i>GAPDH</i>. Blood was processed within 2 hr or 6 hr post-venepuncture and cfDNA isolated from 1 ml of plasma. Whiskers of the box-plot show minimum and maximum values. B. Comparison of miRNA profiles from plasma processed 6 hr post-venepuncture compared to samples processed at 2 hr. Results presented as -ΔC_T (change from 2 hours. Error bar = ±Std Dev, n = 5. *<i>p</i><0.05; **<i>p</i><0.01; ***<i>p</i><0.001. C. Two-tailed Spearman’s correlation analysis of ΔC_T values obtained from TLDA A cards for duplicate miRNA samples following centrifugation at 1000g and 2000g. Dotted lines represent the 95% confidence interval. D. Two-tailed Spearman’s correlation analysis of ΔC_T values obtained from TLDA A cards for duplicate miRNA samples following centrifugation at 1000g and 10000g. Dotted lines represent the 95% confidence interval. E. Two-tailed Spearman’s correlation analysis of ΔC_T values obtained from TLDA A cards for duplicate miRNA samples following centrifugation at 2000g and 10000g. Dotted lines represent the 95% confidence interval. For C - E, Rho (ρ) and <i>p</i>-values are indicated on each graph.