Anatomical and biochemical correlates of dopaminergic function in wild type (WT, <i>white</i><i>bars</i>) and <i>Fmr1</i>^-/Y mice (red bars).

<p><b>A</b>. Mean numbers of tyrosine hydroxylase (TH) expressing neurons (± SEM, <i>top</i>) in the substantia nigra pars compacta (SNc) and ventral tegmental area (VTA) of WT and <i>Fmr1</i>^-/Y mice estimated with design-based stereology. Numbers of mice are indicated in each column. Ventral midbrain image (bottom left) is magnified 4x and inset showing TH staining (bottom right) 40x. Asterisks (*) indicate <i>p</i> < 0.05 vs. WT. cp = cerebral peduncle; fr = fasciculus retroflexus; ml = medial lemniscus. <b>B</b>. Mean ratio of TH to β-Actin staining intensity expressed as percentage of mean WT values (± SEM, <i>top</i>) in western blots of homogenates from dorsal striatum and nucleus accumbens (NAc) of WT and <i>Fmr1</i>^-/Y mice. Numbers of mice are indicated in each column. Representative bands (bottom) are shown for each column above.