Eugenol does not induce leakage of liposomes made of total yeast lipids or inhibit protein synthesis.

<p>(A) Release of carboxyfluorescein from large unilamellar vesicles (LUVs) over a series of eugenol concentrations (0.003 to 1.6 mg/ml, bottom axis) was compared to 100% release from liposome exposed to Triton-X 100. <i>Echinacea purpurea</i> extract was used as a positive lysis control over a concentration gradient of 0.01 to 5% (top axis). Data correspond to the mean % leakage values (±SD) of three independent experiments. (B) Yeast exposed to subinhibitory (0.21 mg/ml) or inhibitory (0.27 mg/ml) concentrations of eugenol do not have significantly decreased β-galactosidase activity in comparison to the untreated control. In contrast, the inhibitor of protein translation, cycloheximide, significantly reduced β-galactosidase activity in the assay. These observations indicate that eugenol does not reduce efficiency of translation in yeast as would be expected for compounds that perturb the intracellular pool of amino acids. The values are expressed as mean ±SD of triplicates, difference between treatment and untreated control are indicated as p<0.05 (*) and p<0.01 (**).