HIV-GFP infection is extremely inefficient in ρ⁰ cells devoid of mtDNA.

<p>(<i>A</i>) Estimation of cellular infectivity by HIV-1 in HOS and ρ⁰ cells. GFP expression in different cells was measured by FACS analysis and was plotted against the amount of virus used in each experiment. (<i>B</i>) Representative histograms of GFP quantification by FACS analysis in cells infected with an equivalent of 20 ng of p24 of HIV-GFP. Gray bar indicates the gate for GFP expression. The graph is representative of three independent experiments.