Microscopic analysis of <i>P. citri</i> PCVAL bacteriocytes showing the distribution of <i>M. endobia</i>’s GroEL and MscL.

<p>(<b>A–D</b>) FISH detection of <i>M. endobia</i> and <i>T. princeps</i> cells in <i>P. citri</i> bacteriocytes. Bacteriocyte nuclear genome is stained with DAPI (A, blue), <i>T. princeps</i> with probe b91 (B, red), <i>M. endobia</i> with probe g630 (C, green). Panel D shows the combination of all staining. (<b>E–J</b>) Immuno-histochemistry detection of GroEL (E–G) and MscL (H–J). Bacteriocyte nuclear genome is stained with DAPI (blue). Images G and J are amplifications of interesting details (in squares) from pictures F and H, respectively. No specific fluorescent signal was observed when serum of unimmunized rabbits was used (negative controls, E and H).