Analysis of single nucleotide polymorphisms in the coding region of hSTING.

<p>(A) Sequence alignment of STING from different species. The four variations in the coding region of hSTING, R71, G230, R232 and R293 are shown in bold and colored in red. The evolutional conservation of R232 and R293 among the species is highlighted. The residue numbers denote those in hSTING. (B) Allele frequency of hSTING. The frequencies derived from 1000 Genome Project database are in the left-most column and the amino acid variations are colored in red. The name of hSTING SNPs and mutants studied in this work are shown in bold and the frequency is calculated in the right-most column. (C) Localization of G230, R232 and R293 in the crystal structure of hSTING in complex with c-di-GMP. A monomer of the hSTING bound to c-di-GMP is shown to allow ease of identification of the residues. Residues G230, R232 and R293 are respectively, colored blue, green and red.