Cytological analysis of control (Ct) and F3.1.1-treated U373 cells.

<p>All treatments with fraction F3.1.1 were done at 4 µg/mL for 72 h. (A) F-actin filament immunostaining with phalloidin conjugated to Alexa Fluor 488 (red) and visualization of nuclei with DAPI staining (blue). Arrows indicate irregularly shaped nuclei. (B) Flow cytometry analysis of the cell size distribution of the entire cell population as measured by electronic volume (EV). (C) Cytoskeleton immunostaining for F-actin (red) and with anti-α/β-tubulin antibodies for the microtubule arrangement (green). The scale is the same in all panels (bar = 20 µm).