Expression of selected miRNAs in tissue samples.

<p>(A) Validation of miRNAs by qRT-PCR: Box plots representing the tissue expression level of four miRNAs in 40 FFPE PCa tissues and their paired adjacent normal tissues. Expression levels of the miRNAs are normalized to U6 snRNA as endogenous control. Statistically significant differences were determined using unpaired Student’s T-test. We detected significant decrease in the expression of miR-205 (<i>p</i><0.0001), miR-214 (<i>p</i><0.0001), miR-221 (<i>p</i><0.001) and miR-99b (<i>p</i><0.0001) in PCa tissues as compared to adjacent normal tissues. *denotes <i>p</i><0.0001 and **denotes <i>p</i><0.001. (B) Receiver operating characteristic (ROC) curve analysis: ROC curve for four miRNAs were made to differentiate PCa from healthy tissues. The area under the ROC curve (AUC) for each miRNA conveys its accuracy for differentiation of PCa tissues and normal tissues in terms of sensitivity and specificity.