Effects of genistein on PSA expression and promoter activities in PCa cells.

<p>A and B: Western blot analysis of the effect of different concentrations of genistein on PSA protein expression in LAPC-4 and LNCaP cells, respectively. C: Signal relative intensity was normalized to β-tubulin. D: Quantitative assessment by real-time PCR of PSA mRNA expression in LAPC-4 and LNCaP cells in response to genistein treatment. E: Effects of genistein on PSA luciferase activity in LAPC-4 and LNCaP cells that were transfected with PSA promoter linked to a luciferase reporter in the pGL3-Basic vector. Results represent the means ± SD of three independent experiments. *p < 0.05, **p <0.01, for comparisons with the untreated control groups.