Characteristics of 3T3-L1 cells with Brd2 knockdown.

<p>3T3-L1 cells were transfected with Brd2 shRNA (SiBrd2) or scramble shRNA (SiC) plasmids respectively. (A) Adipogenic differentiation of 3T3-L1 adipocytes from pre-adipocytes and Oil Red O staining on day 4 of treatment reveals lipids in cells that have undergone differentiation. (B) Quantification of lipid content in transfected 3T3-L1 cells after adipogenic differentiation (n = 3; **<i>P</i> < 0.01). (C) 3T3-L1 cells transfected with Brd2 shRNA (SiBrd2) or scramble shRNA (SiC) plasmids, respectively, cultured without MDI induction for 8 days, and then stained with Oil Red O. (D) Quantification of lipid content in transfected 3T3-L1 cells after adipogenic differentiation (n = 3; **<i>P</i> < 0.01).