<i>in</i><i>vitro</i> limit of quantification (on the left) estimated by reproducible TaqMan real-time PCR amplifications of MAP0865 (n=63) and ext-RD9 (n=21) for MAP K-10 (A) and <i>M. tuberculosis</i> H37Rv quantification (B), and limits of quantification in tissues (on the right) estimated by distribution analyses of sample number according to MAP K-10 (A) and <i>M. tuberculosis</i> H37Rv (B) densities, estimated by TaqMan real-time PCR targeting MAP0865 (n=40 spleen, n=40 liver) or ext-RD9 (n=7 spleen, n=9 lung), respectively.

<p>All these accurate real-time PCR results are observed for a host DNA amount < 3 µg in a final volume of 25 µl, and DNA purity at 260/280 nm ratio = 1.89±0.08.