Histological analysis of <i>M.tb</i>-exposed lungs.

<p>Four Balb/c mice from each group shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078312#pone-0078312-g002" target="_blank">Figure 2C</a> had one lung lobe inflated with 10% NBF and dissected. Lungs were embedded in paraffin, sectioned, mounted onto slides and stained with haematoxylin and eosin. Images were cropped so that no external white space was visible (<b>A</b>). Analysis was performed using a k means clustering algorithm in MATLAB (<b>B</b>). (<b>C</b>) Representative sections from each group. (<b>D</b>) Percentages of the image represented by one of three colours (purple, red or white) were calculated and plotted, n = 2-4.