Influence of ATP and BBG on dye uptake of EYFP/Panx1 WT mutant expressing N2a cells.

<p>N2a cells expressing (A-D) EYFP or EYFP-tagged mPanx1, drPanx1a, drPanx1b or (E, F) drPanx1a-C76R, -C76K, -C76A, -C76E or drPanx1b-R75C, - R75K, -R75A, -R75E mutants were used for dye uptake assays 48 h post transient transfection. ATP or BBG were applied together with EtBr (20 µM). Each bar represents the mean of EtBr fluorescence + SEM of 135 cells analyzed 5 min after EtBr/compound application. All values were normalized to the respective control condition (Ctrl), which was set to 100%. (p<0.001 = ***). (<b>A</b>-<b>D</b>) <i>Influences of ATP and BBG on dye uptake in EYFP or Panx1 expressing N2a cells</i>. The total EtBr uptake in relative fluorescence units is depicted on the right y-axis. In (B)-(D), the basal dye uptake of EYFP expressing cells is depicted as a reference. (<b>E</b>,<b>F</b>) <i>Cross-comparison of the ATP- and BBG-mediated reduction of dye uptake in (E) drPanx1a WT and -C76 mutant as well as in (F) drPanx1b WT and -R75 mutant expressing N2a cells</i>. The respective control condition set to 100% is not depicted. (n = 135 cells per group; p<0.05 = *; p<0.01 = **; p<0.001 = ***).