Leaf disk senescence assay for salinity and drought tolerance in transgenic tobacco plants (T3).

<p>(A) Representative pictures to show phenotypic differences in leaf disks of wild type (WT), vector control (VC) and T3 plants (CD-1 and CD-6) after incubation in 0, 100, 200, 300, 400 and 500 mM NaCl or 500 mM mannitol solutions. (B) Representative diagram to show the chlorophyll content from leaf disks of wild type, vector control and T3 transgenic plants after incubation in 200 mM NaCl or 300 mM mannitol solutions. Data are presented as mean <u>+</u> SE (n=3) and error bars represent SE. Data shows a significant difference of chl.a or chl.b content between transgenic and wild type controls at P<0.0001, by Student’s t-test.