H₂O₂ induces phosphorylation of IGF-1R.

<p>(A) Subconfluent HPAEC were treated with 1 μM and 10 μM H₂O₂ and incubated in a CO₂ incubator. After 20 minutes, whole cell lysates were prepared and western blotting was performed to determine the phosphorylation status of IGF-1R. (B) HPAEC were treated with 5 μM AG1024 for 30 minutes and then with 10 μM H₂O₂ for 20 minutes. Western blotting was then performed to determine phosphorylation status of IGF-1R. (C) HPAEC were treated with 20 mM NAC and subjected to 10 Gy X-rays. Western blotting was then performed 3 hours post-irradiation to determine phosphorylation status of IGF-1R.