Expression of zebavidin in zebrafish.

<p>Relative zebavidin mRNA quantification was performed with qRT-PCR (A) in adult zebrafish tissues and (B) in developing zebrafish (0-7 days post fertilization (dpf)). Expression analysis of each tissue in (A) are shown as the mean (error bar = SD) of the biological replicates. The two-sample t-test was used to compare the expression between female gonads and female gills (P = 0.002), male gonads and male gills (nonsignificant, NS), female gonads and male gonads (P = 0.023) and female gills and male gills (NS). Expression analysis of the developmental samples in (B) are shown as the mean (SD) of the biological replicates on 0 dpf and as the mean (SD) of the technical replicates on 1-7 dpf. All of the developmental samples were pooled from 15 to 50 individuals depending on the age of embryos. The two-sample t-test was used to compare the expression of 0 dpf and 1 dpf samples to later time points. A statistically significant difference in expression was found between the 0 dpf sample and all of the other samples (P < 0.0001 in all comparisons) and also between the 1 dpf sample and the samples of 2, 4, 5, 6 and 7 dpf (P < 0.05 in all comparisons). In both (A) and (B) the expression of zebavidin is normalized to <i>EF1a</i> expression. The insets in the upper right corners represent enlargements of parts of the actual figures. (C) Embryos, injected with a Morpholino blocking the translation initiation site or with a random control Morpholino, 24 h after injection in comparison with WT embryos. (D) Embryos 48 h after injection.