Interspecies PMCA with vCJD macaque brain homogenate and red-backed vole 170N/N as the substrate.

<p>Macaque vCJD brain material and hamster 263K scrapie brain material was diluted 10²-fold into 10% RBV 170N/N NBH and subjected to 1 round of PMCA (lanes 1-4 respectively). Lanes 5-6 indicate control PMCA samples containing 10% RBV 170N/N NBH substrate only without added seed. Scrapie hamster brain material was diluted 10² fold into 10% hamster NBH and subjected to 1 round of PMCA (lanes 7-8). Lanes 9-10 indicate 10% hamster NBH without seed subjected to 1 round of PMCA. Scrapie hamster brain homogenate (1%) was loaded in lanes 11^-12 as a positive control (Cont.). Prior to electrophoresis, samples were digested with PK. The blot was stained with 6D11 antibody.