Islet histology in ß-ARNT and floxed control mothers.

<p>(A–B) Representative images of insulin immunofluorescence on islets from a floxed control (A) and ß-ARNT (B) mouse in late pregnancy. (C) Beta-cell mass in floxed control (FC) (n = 6) and ß-ARNT (n = 6) females in late pregnancy. p = ns, t-test. (D–E) Representative BrdU (red), insulin (green) and DAPI (blue) immunofluorescence on islets from a (D) floxed control and (E) ß-ARNT mouse in late pregnancy. (F) The BrdU labelling index was significantly decreased in insulin-positive islet cells of ß-ARNT versus floxed control (FC) dams (median 1.86 (1.72–2.55) versus 3.96 (1.86–6.6)) *p<0.05, Mann Whitney test). Data points represent individual mice. (G) The caspase labelling index was similar in insulin-positive islet cells of ß-ARNT versus floxed control dams (median 1.69 (0.48–1.26) versus 0.84 (0.56–3.01) p = NS, Mann Whitney test). Data points represent individual mice.