The expression of the <i>nrf</i> gene family during development as measured by quantitative real-time PCR.

<p>Black lines indicate expression pre-hatch and grey lines indicate expression post-hatch. Values were normalized to the 120 hpf time-point and <i>β-actin 1</i> (actb1) was used as the housekeeping gene. Data are presented as the mean ± S.E.M. (error bars), and <i>N</i> = 4 pools of 30 embryos. Differences in expression between hatching state were assessed using ANOVA followed by Fisher's PLSD (*, <i>p</i> ≤ 0.05). Data for expression of <i>nrf2a</i> and <i>nrf2b</i> are from Timme-Laragy et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079574#B7" target="_blank">7</a>] and are used with permission of the American Society for Biochemistry and Molecular Biology. Previously these data were presented as molecule number. Here we have normalized the data to β-actin 1 expression and to the 120 hpf time point and re-plotted for comparison to the new data on expression of other <i>nrf</i> family genes. Dashed lines indicate the value of 1.0 from the 120 hpf time point.