TCE treatment inhibits cell proliferation and induces process outgrowth.

<p>(Table 1) Phytochemical analysis of TCE. (<b>A</b>) MTT assay showing dose dependent decrease in cell number in TCE treated U87MG, HeLa, PC3 and C6 cells. Graph showing IC₅₀ for U87MG, HeLa and C6 cells at 200 μg/ml and for PC3 cells at 500 μg/ml. (<b>B</b>) MTT assay showing the effect of hexane and chloroform fractions on C6 glioma cells. (<b>C</b>) Bright field images of cells stained with 1% toluidine blue and 1% methylene blue after fixing with glutaraldehyde. Scale bar-200 μm. (<b>D</b>) Histogram representing length of total and individual cell processes in TCE treated and control cells. At least 100 cells from each sample in every experiment were counted for process outgrowth analysis. (<b>E</b>) Histogram showing average of number of processes in TCE treated and untreated cells. Values are representative of mean ± SEM. ‘*’ (<i>P</i><0.05) and ‘**’ (<i>p</i>< 0.01) represent statistical significant difference between control and TCE treated groups.