Sarcomere Length and Calcium Transient Measurements in Tamoxifen-Treated Rat Cardiac Myocytes.

<p>A) Representative traces of sarcomere shortening normalized to baseline. B-D) Sarcomere length data was collected from myocytes treated with Tam and paced at 0.2 Hz and 36 ± 1°C. Data are from four rat cardiac myocyte preparations, N = 46-52 cells/treatment. E) Representative traces of Ca²⁺ transients normalized to baseline. F-H) Myocytes were treated with Fura-2AM, and Ca²⁺ transient data was collected at 0.5 Hz and 36 ± 1°C after Tam treatment. Data are from three rat cardiac myocyte preparations, N = 11-30 cells/treatment. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 compared to 0 µM.