Twist 1 promotes FoxM1 promoter activity through recruiting p300.

<div><p>(A) Diagram of the Twist 1-binding site in the human FoxM1 promoter (1kb) and two FoxM1 luciferase reporters. WT-Luc: wild-type luciferase reporter; Mut-Luc: luciferase reporter carrying point mutations of Twist 1 binding site. Mutations were underlined.</p> <p>(B) Activation of FoxM1 luciferase reporters by Twist 1 in NCI-N87 cells.</p> <p>(C) The binding of Twist 1 on the promoter regions of human FoxM1 gene were analyzed by ChIP assays and quantified by real-time PCR. The region containing -2000 to -1800 bp were used as a negative control. </p> <p>(D) Co-immunoprecipitation of p300 and Twist 1 in NCI-N87 cells.</p> <p>(E) Activation of FoxM1 luciferase reporters by Twist 1 and p300 in NCI-N87 cells.</p> <p>(F) Representative western blot analysis of p300 expression in NCI-N87 cells transfected with siRNA targeting p300 or negative control (Ctrl).</p> <p>(G-H) Quantitative real-time PCR (G) and western blot (H) analysis of FoxM1 expression in NCI-N87 cells transfected with adenoviruses expressing empty vector (EV) or Twist 1. Cells were pre-transfected with siRNA oligos targeting p300 or negative control siRNA (Ctrl) for 24 hours.</p>