Twist 1 depletion inhibits gastric cell proliferation.

<div><p>(A-D) Quantitative real-time PCR and western blot analysis of Twist 1 expression in NCI-N87 (A-B) or AGS (C-D) cells transfected with siRNA oligos targeting Twist 1 or negative control siRNA (Ctrl).</p> <p>(E-F) The growth curve of NCI-N87 (E) or AGS (F) cells cells transfected with siRNA oligos targeting Twist 1 or negative control siRNA (Ctrl).</p> <p>(G-H) The cell proliferative potential (BrdU) was determined in NCI-N87 (G) or AGS (H) cells transfected with siRNA oligos targeting Twist 1 or negative control siRNA (Ctrl).</p> <p>(I-J) The cell cycle phase of NCI-N87 (I) and AGS (J) cells transfected with siRNA oligos targeting Twist 1 or scramble siRNA (Ctrl) were analyzed by flow cytometry. </p>